7PZE

MademoiseLLE domain 2 of Rrm4 from Ustilago maydis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979340
Spacegroup name	P 43 21 2
Unit cell lengths	53.455, 53.455, 144.873
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.010 - 2.600
R-factor	0.2271
Rwork	0.225
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	own model
RMSD bond length	0.009
RMSD bond angle	0.977
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.510	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	6898	665
<I/σ(I)>	17.9	2.79
Completeness [%]	98.3
Redundancy	7.3
CC(1/2)	0.997	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	285	0.2 M potassium chloride, 20 % (w/v) PEG 3350