7PZ8

Structure of an LPMO at 3.12x10^6 Gy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-03
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	34.410, 87.270, 37.390
Unit cell angles	90.00, 104.99, 90.00

Refinement procedure

Resolution	43.630 - 1.400
R-factor	0.1521
Rwork	0.151
R-free	0.16890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zud
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.400	1.400
Number of reflections	75532	5685
<I/σ(I)>	8.83
Completeness [%]	91.1
Redundancy	1.53
CC(1/2)	0.998	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20 mM MgCl2, 0.1 M HEPES pH 7.5, 22 %(w/v) Polyacrylic acid 5100 sodium salt.