7PZ2
Structure of the mechanosensor domain of Wsc1 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.820, 53.500, 51.930 |
| Unit cell angles | 90.00, 95.46, 90.00 |
Refinement procedure
| Resolution | 28.230 - 1.580 |
| R-factor | 0.1518 |
| Rwork | 0.150 |
| R-free | 0.18360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fwt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.750 | 1.639 |
| High resolution limit [Å] | 1.583 | 1.583 |
| Rmerge | 0.063 | 0.465 |
| Rmeas | 0.076 | 0.568 |
| Rpim | 0.042 | 0.320 |
| Number of reflections | 23125 | 2133 |
| <I/σ(I)> | 10.35 | 2.17 |
| Completeness [%] | 97.1 | 91.42 |
| Redundancy | 3 | 2.8 |
| CC(1/2) | 0.997 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 278 | 80 mM sodium acetate, 1.6 M ammonium sulfate, 20% glycerol, 70 mg/ml ScWsc1 |
