7PYN

Structure of an LPMO (expressed in E.coli) at 2.31x10^5 Gy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-03
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.033
Spacegroup name	P 41
Unit cell lengths	48.930, 48.930, 108.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 1.400
R-factor	0.1433
Rwork	0.142
R-free	0.16080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pqr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.400	1.400
Number of reflections	91816	6541
<I/σ(I)>	10
Completeness [%]	92.2
Redundancy	2.02
CC(1/2)	0.990	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4.5	278	2.3 M ammonium sulfate, 0.1 M sodium acetate pH 4.5 under Paraffin:Silicon oil in a 3:2 ratio