7PYI

Structure of LPMO in complex with cellotetraose at 6.65x10^6 Gy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.033
Spacegroup name	P 41 3 2
Unit cell lengths	126.010, 126.010, 126.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.100 - 2.050
R-factor	0.2204
Rwork	0.218
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ach
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.100	2.100
High resolution limit [Å]	2.050	2.050
Number of reflections	21166	1596
<I/σ(I)>	15.87
Completeness [%]	95.9
Redundancy	3.29
CC(1/2)	0.990	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	298	3.3 M NaCl, 0.1 M Citric acid pH 3.5