7PXS
Room temperature X-ray structure of LPMO at 1.91x10^3 Gy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2021-10-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 126.630, 126.630, 126.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.250 - 1.900 |
| R-factor | 0.1708 |
| Rwork | 0.169 |
| R-free | 0.19560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ach |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.800 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 27939 | 2033 |
| <I/σ(I)> | 8.67 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.9 | |
| CC(1/2) | 0.990 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | 3.0 M NaCl, 0.1 M Citric acid pH 3.5 |
