Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-08-12 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.54190 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 79.507, 79.507, 138.224 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.322 - 2.180 |
| Rwork | 0.206 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dai |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.769 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.322 | 38.290 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.059 | 0.016 |
| Rmeas | 0.081 | 0.022 |
| Rpim | 0.054 | 0.015 |
| Number of reflections | 13347 | 13347 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 95.2 | 95.2 |
| Redundancy | 2.4 | 2.2 |
| CC(1/2) | 0.997 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M BisTris pH 6-7, 1.7-2.1M Ammonium sulphate |
