7PX0
Drosophila melanogaster Aldehyde Oxidase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 144.663, 127.914, 152.547 |
| Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
| Resolution | 48.660 - 2.200 |
| R-factor | 0.1772 |
| Rwork | 0.175 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3unc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.813 |
| Data reduction software | XDS |
| Data scaling software | Aimless (1.10.28) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.660 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 257441 | 12300 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.986 | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | PEG 3350, ammonium citrate, dithiothreitol |
