7PWJ

dUTPase from human in complex with Stl

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-11-24
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.99186
Spacegroup name	P 21 21 21
Unit cell lengths	77.720, 81.797, 198.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.199 - 1.944
R-factor	0.21659
Rwork	0.214
R-free	0.26191
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h49 1q5u
RMSD bond length	0.008
RMSD bond angle	1.485
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.200	1.995
High resolution limit [Å]	1.944	1.944
Number of reflections	92354	5386
<I/σ(I)>	18.9
Completeness [%]	98.5
Redundancy	12.9
CC(1/2)	0.999	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	1.6M ammonium sulphate and 1M lithium sulphate.