7PWH

Structure of the dTDP-sugar epimerase StrM

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9159
Spacegroup name	C 2 2 21
Unit cell lengths	42.376, 131.542, 78.539
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.420 - 1.900
Rwork	0.201
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hmz
RMSD bond length	0.006
RMSD bond angle	1.401
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.420	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	17624	1122
<I/σ(I)>	5.5
Completeness [%]	99.4
Redundancy	4.3
CC(1/2)	0.991	0.312

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	10% PEG 6000, 30% PEG 300, 100 mM CaCl2, 50 mM Tris pH 7.0 + 10 mM dTDP