7PVK
X-ray structure of dimeric PorX (T272A mutant), in complex with pGpG.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.630, 103.770, 132.210 |
| Unit cell angles | 90.00, 98.50, 90.00 |
Refinement procedure
| Resolution | 51.900 - 2.400 |
| Rwork | 0.194 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.900 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.033 | 0.948 |
| Number of reflections | 98575 | 9713 |
| <I/σ(I)> | 15.93 | |
| Completeness [%] | 99.4 | 98.28 |
| Redundancy | 14.4 | 14.3 |
| CC(1/2) | 0.999 | 0.635 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Glycine pH 8.5, 100 mM sodium formate, 3-4 % PEG 8 K, 15 mM MgCl2, 30 mM NaF, 5 mM BeSO4, 0.5 mM ZnCl2, 0.5 mM pGpG |
