7PVA

1.9 Angstrom crystal structure of dimeric PorX, co-crystallized in the presence of zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.28202
Spacegroup name	P 1
Unit cell lengths	61.480, 77.290, 138.830
Unit cell angles	82.42, 81.61, 76.51

Refinement procedure

Resolution	68.590 - 1.910
Rwork	0.159
R-free	0.19200
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.590	1.980
High resolution limit [Å]	1.910	1.910
Rpim	0.038	0.718
Number of reflections	186912	18467
<I/σ(I)>	12.29
Completeness [%]	98.3	96.75
Redundancy	16.9	17
CC(1/2)	0.998	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	100 mM glycine pH 8.0, 100 mM sodium formate and 4% PEG 8000