7PUZ

Crystal structure of the Mic60 coiled coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 4 21 2
Unit cell lengths	54.194, 54.194, 134.042
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.681 - 2.842
R-factor	0.2466
Rwork	0.244
R-free	0.28960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	idealized alpha helices
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMPLE
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.681	44.681	3.010
High resolution limit [Å]	2.840	8.390	2.840
Rmerge	0.161	0.036	2.542
Rmeas	0.168	0.038	2.638
Total number of observations	69737
Number of reflections	5166	255	801
<I/σ(I)>	13.11	50.74	0.99
Completeness [%]	99.7	98.5	99
Redundancy	13.499	10.549	14.02
CC(1/2)	0.999	0.999	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	17.5 % PEG 1500, 0.1 M MMT buffer pH 7.1, 0.1 M D-Sorbitol