7PT5

Crystal structure of the CH domain of human CEP44

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-06-23
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97928
Spacegroup name	P 41 21 2
Unit cell lengths	33.949, 33.949, 243.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.620 - 2.300
R-factor	0.2299
Rwork	0.226
R-free	0.26450
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.558
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.100	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.084
Rpim	0.019	0.506
Number of reflections	7159	661
<I/σ(I)>	17.7	1.9
Completeness [%]	100.0	100
Redundancy	21.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292.15	100 nl protein solution and 100 nl of reservoir solution which was 0.1 M Hepes pH 7.3, 10% isopropanol, 10% PEG-4000. Crystals were mounted 0.1 M Hepes pH 7.5, 7.5% isopropanol, 30% PEG-400.