7PRP

Crystal Structure of the B subunit of heat labile enterotoxin LT-IIc from Escherichia coli in apo form

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	73.402, 73.688, 102.354
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.981 - 2.300
Rwork	0.196
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5g3l
RMSD bond length	0.015
RMSD bond angle	1.599
Data reduction software	XDS
Data scaling software	Aimless (0.5.032)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	2.390
High resolution limit [Å]	2.300	2.300
Rmerge	0.096	0.455
Rmeas	0.125	0.597
Rpim	0.079	0.382
Number of reflections	24817	9279
<I/σ(I)>	8.1	2.5
Completeness [%]	99.6	97.2
Redundancy	3.7	3.6
CC(1/2)	0.996	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	292	2.4 sodium formation and 100 mM citric acid pH 4.0.