7PRC

PHOTOSYNTHETIC REACTION CENTER FROM RHODOPSEUDOMONAS VIRIDIS (DG-420315 (TRIAZINE) COMPLEX)

Experimental procedure

Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	263
Detector technology	IMAGE PLATE
Collection date	1993-08
Detector	MARRESEARCH
Wavelength(s)	0.92, 1.0
Spacegroup name	P 43 21 2
Unit cell lengths	223.500, 223.500, 113.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.650
R-factor	0.19 *
Rwork	0.184
R-free	0.23000 *
Structure solution method	DIFFERENCE FOURIER / SA OMIT MAPS
Starting model (for MR)	6prc
RMSD bond length	0.012
RMSD bond angle	1.520 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.670
High resolution limit [Å]	2.600	2.600
Rmerge	0.074	0.279 *
Total number of observations	352617 *
Number of reflections	72314 *
<I/σ(I)>	7.4	0.8
Completeness [%]	88.3 *	77.8 *
Redundancy	4.6	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	1.5 (M)
2	1	reservoir	ammonium sulfate	3 (M)	or 2.6M or 2.4M