7PPY

CARM1 in complex with EML709

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 2
Unit cell lengths	74.677, 98.823, 206.981
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.060 - 2.420
R-factor	0.207
Rwork	0.205
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.060	2.490
High resolution limit [Å]	2.420	2.420
Rmerge	0.115	1.223
Rmeas	0.131	1.441
Rpim	0.062	0.743
Number of reflections	58136	3623
<I/σ(I)>	8.8	0.9
Completeness [%]	98.3	80
Redundancy	4.3	3.2
CC(1/2)	0.998	0.441

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	12% PEG 2000, 100mM NaCl, 100mM TrisHCl pH8