7PPP

Crystal structure of ZAD-domain of ZNF_276 protein from rabbit.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.87313, 1.282
Spacegroup name	P 63 2 2
Unit cell lengths	82.940, 82.940, 134.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.100 - 2.900
Rwork	0.290
R-free	0.33990
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	2.922
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.100	49.100	3.000
High resolution limit [Å]	2.900	10.000	2.900
Rmerge	0.052	0.024	1.363
Rmeas	0.054	0.026	1.420
Total number of observations	71337
Number of reflections	6460	191	609
<I/σ(I)>	25.91	70.15	1.87
Completeness [%]	98.8	90.5	99.8
Redundancy	11.043	8.188	11.586
CC(1/2)	0.999	0.998	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1M bicine pH 8.5, PEG 20000 5%, dextrane sulfate 5%