7PON
C TERMINAL DOMAIN OF NIPAH VIRUS PHOSPHOPROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07189 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 35.319, 35.319, 112.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.710 - 2.100 |
| R-factor | 0.22987 |
| Rwork | 0.225 |
| R-free | 0.27314 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.841 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.630 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.033 | 0.398 |
| Rpim | 0.019 | 0.234 |
| Number of reflections | 4643 | 2364 |
| <I/σ(I)> | 23.6 | 3.3 |
| Completeness [%] | 99.6 | 98.6 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M BIS-TRIS, 25% PEG 3350, 0.2M MAGNESIUM CHLORIDE, PH 6.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
