7POJ
Prodomain bound BMP10 crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91188 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 143.351, 144.108, 82.129 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.630 - 3.500 |
| R-factor | 0.2758 |
| Rwork | 0.275 |
| R-free | 0.29980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7poi |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.487 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.630 | 3.830 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.169 | 2.702 |
| Rmeas | 0.180 | 2.880 |
| Rpim | 0.062 | 0.981 |
| Number of reflections | 11092 | 2583 |
| <I/σ(I)> | 6.3 | 0.7 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 8.5 | 8.5 |
| CC(1/2) | 0.996 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 294.15 | 20% PEG3350 0.2 M ammonium tartrate dibasic |
