7POI

Prodomain bound BMP10 crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91589
Spacegroup name	C 2 2 21
Unit cell lengths	72.897, 214.041, 106.967
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	107.020 - 2.900
R-factor	0.2209
Rwork	0.218
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sf3 4yci
RMSD bond length	0.002
RMSD bond angle	0.470
Data reduction software	DIALS (1.14.5)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.020	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.166	3.761
Rmeas	0.173	3.919
Rpim	0.048	1.094
Number of reflections	19024	3033
<I/σ(I)>	10.1	0.5
Completeness [%]	100.0	100
Redundancy	12.9	12.8
CC(1/2)	1.000	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	294.15	19% PEG 3350 0.15 M Ammonium tartrate dibasic 0.02 M Sodium cacodylate trihydrate