7POI
Prodomain bound BMP10 crystal form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91589 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 72.897, 214.041, 106.967 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 107.020 - 2.900 |
R-factor | 0.2209 |
Rwork | 0.218 |
R-free | 0.26840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sf3 4yci |
RMSD bond length | 0.002 |
RMSD bond angle | 0.470 |
Data reduction software | DIALS (1.14.5) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.020 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.166 | 3.761 |
Rmeas | 0.173 | 3.919 |
Rpim | 0.048 | 1.094 |
Number of reflections | 19024 | 3033 |
<I/σ(I)> | 10.1 | 0.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.9 | 12.8 |
CC(1/2) | 1.000 | 0.482 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 294.15 | 19% PEG 3350 0.15 M Ammonium tartrate dibasic 0.02 M Sodium cacodylate trihydrate |