7PNR

Human Angiogenin mutant-S28AT36AS37A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 2
Unit cell lengths	84.632, 37.342, 39.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.320 - 1.600
R-factor	0.19038
Rwork	0.190
R-free	0.20103
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b1i.fasta
RMSD bond length	0.013
RMSD bond angle	1.588
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.630	1.550
High resolution limit [Å]	1.520	1.520
Number of reflections	19602	628
<I/σ(I)>	19.7
Completeness [%]	97.9
Redundancy	5
CC(1/2)	0.999	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Malic acid/NaOH pH 7.0, PEG 3350