7PNN

mVenus released from fusion protein.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	61.064, 91.048, 118.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.500 - 1.430
R-factor	0.1619
Rwork	0.162
R-free	0.16890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ndj
Data reduction software	XDS (BUILT=20210323)
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3.)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.280	1.520
High resolution limit [Å]	1.430	1.430
Rmeas	0.074	2.159
Number of reflections	60635	9593
<I/σ(I)>	18.81	1.52
Completeness [%]	99.0	97.8
Redundancy	13.36	13.56
CC(1/2)	0.999	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	200 mM KCl, 20% (w/v) PEG3350