7PMQ

DEAD-box helicase DbpA in the active conformation bound to a hairpin loop RNA and ADP/BeF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	159.644, 159.644, 204.682
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.420 - 3.220
R-factor	0.2294
Rwork	0.228
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2DB3 and 3MOJ
RMSD bond length	0.003
RMSD bond angle	0.587
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.19.2)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.420	3.330
High resolution limit [Å]	3.220	3.220
Rmerge	0.245	1.790
Rmeas	0.255	1.860
Number of reflections	43424	4266
<I/σ(I)>	10.7	1.42
Completeness [%]	99.7	99.65
Redundancy	13.6
CC(1/2)	0.999	0.553

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	200 mM NH4/tartrate 100 mM Tris, pH 8.0 20% (w/v) PEG3350