7PMO
Ruminococcus gnavus ATC29149 endo-beta-1,4-galactosidase (RgGH98)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.313, 85.566, 112.628 |
| Unit cell angles | 90.00, 98.94, 90.00 |
Refinement procedure
| Resolution | 104.030 - 2.100 |
| R-factor | 0.1765 |
| Rwork | 0.175 |
| R-free | 0.20630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d6c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.313 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 104.250 | 104.030 | 2.140 |
| High resolution limit [Å] | 2.100 | 11.500 | 2.100 |
| Rmerge | 0.135 | 0.059 | 0.858 |
| Rmeas | 0.159 | 0.071 | 1.016 |
| Rpim | 0.083 | 0.039 | 0.538 |
| Total number of observations | 2424 | 19825 | |
| Number of reflections | 115358 | 768 | 5694 |
| <I/σ(I)> | 5.4 | 12.8 | 1.4 |
| Completeness [%] | 99.9 | 99.9 | 100 |
| Redundancy | 3.5 | 3.2 | 3.5 |
| CC(1/2) | 0.988 | 0.982 | 0.342 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0% PEG 500 MME, 10% PEG20K, 0.1M Sodium HEPES/MOPS pH 7.5 |
