7PK1
Bovine Glycine N-Acyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2020-10-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.176, 96.176, 144.629 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.090 - 1.650 |
| R-factor | 0.1706 |
| Rwork | 0.169 |
| R-free | 0.19550 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.743 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.090 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.660 |
| Rmerge | 0.044 | |
| Rmeas | 0.046 | |
| Rpim | 0.015 | |
| Number of reflections | 87439 | 2764 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 97.8 | |
| Redundancy | 8.6 | |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 1.5 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6 |
