7PJB
Crystal structure of YTHDC1 with compound PSI_DC1_004
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-18 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.550, 103.060, 42.100 |
Unit cell angles | 90.00, 105.15, 90.00 |
Refinement procedure
Resolution | 40.640 - 1.900 |
R-factor | 0.1879 |
Rwork | 0.185 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3h |
RMSD bond length | 0.008 |
RMSD bond angle | 0.949 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.640 | 2.010 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 25605 | 4095 |
<I/σ(I)> | 8.05 | |
Completeness [%] | 99.6 | |
Redundancy | 3.42 | |
CC(1/2) | 0.993 | 0.512 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5 |