7PJ8

Crystal structure of YTHDC1 with compound DHU_DC1_225

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.560, 103.070, 42.170
Unit cell angles	90.00, 106.01, 90.00

Refinement procedure

Resolution	40.530 - 1.400
R-factor	0.1955
Rwork	0.194
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.006
RMSD bond angle	0.875
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.540	1.490
High resolution limit [Å]	1.400	1.400
Number of reflections	61197	9612
<I/σ(I)>	12.69
Completeness [%]	96.6
Redundancy	3.44
CC(1/2)	0.999	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5