7PI6

Trypanosoma brucei ISG65 bound to human complement C3d

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9655
Spacegroup name	P 1 21 1
Unit cell lengths	51.947, 189.578, 73.880
Unit cell angles	90.00, 90.11, 90.00

Refinement procedure

Resolution	94.790 - 2.600
R-factor	0.2698
Rwork	0.268
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3d
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (20-APR-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.790	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	43218	4392
<I/σ(I)>	3.6
Completeness [%]	98.7
Redundancy	2.9
CC(1/2)	0.875	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES pH 6, 0.2 M MgCl2, 20% PEG 6000 with sliver bullet HR2-996-55