7PGK

HHIP-N, the N-terminal domain of the Hedgehog-Interacting Protein (HHIP), in complex with glycosaminoglycan mimic SOS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9762
Spacegroup name	P 42 21 2
Unit cell lengths	60.240, 60.240, 108.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.000 - 2.750
R-factor	0.268
Rwork	0.267
R-free	0.28600
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.200	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.113
Rpim	0.035
Number of reflections	5609	534
<I/σ(I)>	12	1.1
Completeness [%]	100.0	100
Redundancy	11.9	10.9
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M imidazole/MES pH 6.5, 0.1 M carboxylic acids (0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M sodium citrate, 0.02 M sodium oxamate and 0.02 M potassium sodium tartrate), 10% w/v PEG 20000 and 20% v/v PEG MME 550