7PGI
NaVAb1p (bicelles)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-01 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 178.180, 191.800, 192.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.993 - 3.638 |
| R-factor | 0.2879 |
| Rwork | 0.287 |
| R-free | 0.30350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7pgg |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.980 | 3.769 |
| High resolution limit [Å] | 3.638 | 3.640 |
| Rmerge | 0.157 | 1.000 |
| Number of reflections | 36662 | 3616 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 98.4 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.995 | 0.172 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Sodium acetate, 0.1 M HEPES pH 7.5, 20% PEG 3000, 8% Bicelles |
