7PGG
NaVAb1p detergent (DM)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-19 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.115869, 0.97698, 0.97953, 0.95368 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 157.471, 157.471, 106.394 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.960 - 2.850 |
| R-factor | 0.2669 |
| Rwork | 0.266 |
| R-free | 0.27810 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.092 |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.953 |
| High resolution limit [Å] | 2.850 | 2.852 |
| Rmerge | 0.169 | |
| Number of reflections | 31433 | 3087 |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 99.0 | |
| Redundancy | 16.2 | |
| CC(1/2) | 0.999 | 0.473 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | SeMet labelled NavAb1 purified in DM was concentrated to 13 mg mL-1 and crystallized in 3% PEG300, 0.75M ammonium sulfate. |
