7PGE

copper transporter PcoB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	173
Detector technology	PIXEL
Collection date	2017-05-26
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	65.489, 75.538, 91.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.530 - 2.000
R-factor	0.2221
Rwork	0.221
R-free	0.25310
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.076
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.530	2.043
High resolution limit [Å]	1.970	1.972
Number of reflections	30731	4965
<I/σ(I)>	11.47
Completeness [%]	99.3
Redundancy	6.6
CC(1/2)	0.990	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG4000, NaSCN, sodium acetate