7PG8
NaV_Ae1/Sp1CTD_pore-ANT05 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 |
| Unit cell lengths | 127.175, 127.175, 445.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 4.500 |
| R-factor | 0.3056 |
| Rwork | 0.303 |
| R-free | 0.35170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hk7 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 122.300 | 4.620 |
| High resolution limit [Å] | 4.460 | 4.460 |
| Rmerge | 0.031 | 1.000 |
| Number of reflections | 42377 | 3816 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 98.3 | |
| Redundancy | 2 | |
| CC(1/2) | 0.997 | 0.442 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 13% PEG4000, 2% propanol, 0.1M LiS04, 0.1 M 2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid (ADA) pH 6.5 |
