7PFR
Crystal Structure of Two-Domain Laccase mutant M199A from Streptomyces griseoflavus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97718 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.980, 94.120, 119.730 |
Unit cell angles | 90.00, 91.16, 90.00 |
Refinement procedure
Resolution | 47.240 - 1.750 |
R-factor | 0.1318 |
Rwork | 0.131 |
R-free | 0.16930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lhl |
RMSD bond length | 0.006 |
RMSD bond angle | 0.781 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmeas | 1.190 | |
Number of reflections | 167091 | 12290 |
<I/σ(I)> | 14.65 | 1.53 |
Completeness [%] | 99.0 | |
Redundancy | 6.79 | |
CC(1/2) | 0.990 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | 15 % v/v PEG Smear High, 0.15 M Ammonium acetate, 0.1 M Sodium citrate condition #31 of BCS-1 from Molecular Dimensions, UK |