7PEG

Structure of the sporulation/germination protein YhcN from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	273
Detector technology	PIXEL
Collection date	2018-11-14
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	31.480, 52.280, 134.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.270 - 1.770
R-factor	0.2066
Rwork	0.205
R-free	0.23420
Structure solution method	AB INITIO PHASING
RMSD bond length	0.009
RMSD bond angle	1.193
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Arcimboldo
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.270	41.270	1.870
High resolution limit [Å]	1.770	5.260	1.770
Rmerge	0.066	0.052	0.557
Rmeas	0.074	0.060	0.626
Number of reflections	22053	927	3302
<I/σ(I)>	13.16	23.79	2.75
Completeness [%]	98.3	97	98.4
Redundancy	4.63	4.29	4.76
CC(1/2)	0.994	0.989	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM HEPES pH 7.5, 20% (w/v) PEG 8,000