7PE4
Crystal structure of Mycobacterium hassiacum glucosyl-3-phosphoglycerate synthase at pH 5.5 in complex with UDP-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96112 |
Spacegroup name | I 41 |
Unit cell lengths | 101.887, 101.887, 122.098 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.580 - 2.050 |
R-factor | 0.1618 |
Rwork | 0.160 |
R-free | 0.19200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7p8g |
RMSD bond length | 0.006 |
RMSD bond angle | 0.808 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.085 | 0.973 |
Rmeas | 0.092 | 1.052 |
Rpim | 0.035 | |
Number of reflections | 38917 | 3768 |
<I/σ(I)> | 14.7 | 1.8 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 6.9 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-Tris pH 5.5; 3 M NaCl |