7PDV

Crystal structure of RBM5 RRM1-zinc finger in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.27982
Spacegroup name	P 1 21 1
Unit cell lengths	51.230, 65.890, 94.910
Unit cell angles	90.00, 90.05, 90.00

Refinement procedure

Resolution	47.450 - 3.490
R-factor	0.3421
Rwork	0.337
R-free	0.39000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pcv
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.450	47.450	3.590
High resolution limit [Å]	3.490	15.630	3.490
Rmerge	0.288	0.069	0.928
Rmeas	0.397	0.096	1.268
Total number of observations	25689
Number of reflections	14244	143	1021
<I/σ(I)>	1.9	4.35	0.86
Completeness [%]	90.7	82.7	89.7
Redundancy	1.803	1.825	1.825
CC(1/2)	0.940	0.986	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.05M Sodium cacodylate, 18mM CaCl2, 2.7 mM spermine, 9% 2-propanol