7PDO
Crystal structure of Mycobacterium hassiacum glucosyl-3-phosphoglycerate synthase at pH 5.5 in complex with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96112 |
| Spacegroup name | I 41 |
| Unit cell lengths | 101.338, 101.338, 122.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.570 - 1.970 |
| R-factor | 0.1829 |
| Rwork | 0.182 |
| R-free | 0.19540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7p8g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.852 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.059 | 0.881 |
| Rmeas | 0.064 | 0.957 |
| Rpim | 0.024 | 0.371 |
| Number of reflections | 43448 | 4232 |
| <I/σ(I)> | 17.1 | 1.7 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1 M Bis-Tris pH 5.5; 3 M NaCl |
