7PDA
Crystal structure of Phenazine 1-carboxylic acid decarboxylase from Mycobacterium fortuitum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-03-03 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 3 1 2 |
| Unit cell lengths | 124.696, 124.696, 68.846 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.120 - 2.650 |
| R-factor | 0.1881 |
| Rwork | 0.184 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7abn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.010 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.420 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 25728 | 1284 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.4 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.06 M Divalents, 0.1 M Buffer System 2 pH 7.5, 30% Precipitant mix 3 |
