7PCR

Helicobacter pylori RNase J

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	I 41 2 2
Unit cell lengths	158.490, 158.490, 214.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.030 - 2.750
R-factor	0.2467
Rwork	0.245
R-free	0.26720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bk2
RMSD bond length	0.008
RMSD bond angle	1.212
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.030	2.848
High resolution limit [Å]	2.750	2.750
Rmerge	0.137
Rpim	0.081
Number of reflections	35677	3497
<I/σ(I)>	5.9
Completeness [%]	99.9
Redundancy	3.6
CC(1/2)	0.979	0.453

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	0.1 M HEPES pH 7.5, 25% polyethylene glycol 1000.