7PC4

The PDZ domain of SNTB1 complexed with the PDZ-binding motif of HTLV1-TAX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	54.270, 61.730, 145.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.035 - 2.300
R-factor	0.2077
Rwork	0.205
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 2vrf
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.035	2.360
High resolution limit [Å]	2.300	2.300
Rmeas	0.112	2.244
Number of reflections	22343	1644
<I/σ(I)>	16.58	1.18
Completeness [%]	99.4
Redundancy	13.15
CC(1/2)	0.999	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES pH 7.5, 10% w/v PEG 8000, 8% v/v Ethylene glycol