7PB8

Crystal structure of the CENP-OPQUR complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	199.614, 51.299, 109.999
Unit cell angles	90.00, 117.49, 90.00

Refinement procedure

Resolution	25.650 - 3.680
R-factor	0.2608
Rwork	0.256
R-free	0.35210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qlf
RMSD bond length	0.004
RMSD bond angle	0.754
Data reduction software	MOSFLM
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.300	3.900
High resolution limit [Å]	3.680	3.680
Rmerge	0.084	0.350
Number of reflections	6104	6104
<I/σ(I)>	7.6
Completeness [%]	77.5
Redundancy	2.5
CC(1/2)	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	15% PEG2kmme, 40 mM NaFormate, 200 mM bis-tris propane pH 6.9