7PB6

Structure of LPMO domain of colonization factor GbpA from Vibrio cholerae in the presence of potassium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-04
Detector	MAR CCD 130 mm
Wavelength(s)	0.9537
Spacegroup name	P 43
Unit cell lengths	46.580, 46.580, 157.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.090 - 1.500
R-factor	0.1655
Rwork	0.164
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xwx
RMSD bond length	0.007
RMSD bond angle	0.804
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.090	1.590
High resolution limit [Å]	1.500	1.500
Number of reflections	52629	7798
<I/σ(I)>	28.48	8.74
Completeness [%]	98.5	90.8
Redundancy	13.4	10.9
CC(1/2)	0.999	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Potassium thiocyanate, 30 % w/v PEG 2000 MME