7PAB

Varicella zoster Orf24-Orf27 nuclear egress complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97627
Spacegroup name	P 1 21 1
Unit cell lengths	76.010, 35.050, 158.220
Unit cell angles	90.00, 93.69, 90.00

Refinement procedure

Resolution	19.960 - 2.100
R-factor	0.2181
Rwork	0.215
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zxs
RMSD bond length	0.002
RMSD bond angle	0.489
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	2.175
High resolution limit [Å]	2.100	2.100
Rmeas	0.143	3.780
Rpim	0.041	1.050
Number of reflections	49198	4890
<I/σ(I)>	10.2	0.72
Completeness [%]	99.0	99.53
Redundancy	11.9	12.9
CC(1/2)	0.999	0.463

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	Bis-Tris, ammonium sulfate, PEG 3350, formamide