7P9Q
Crystal structure of Indole 3-Carboxylic acid decarboxylase from Arthrobacter nicotianae FI1612 in complex with co-factor prFMN.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-07-22 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.200, 195.450, 105.210 |
| Unit cell angles | 90.00, 116.35, 90.00 |
Refinement procedure
| Resolution | 43.420 - 2.530 |
| R-factor | 0.1858 |
| Rwork | 0.184 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7abo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.420 | 2.570 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Number of reflections | 123931 | 6258 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.994 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 Carboxylic acid, 0.1 M Buffer System pH 6.5, 30% Precipitant mixture 3 |
