7P9J

Prim-Pol Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137 - Primer Initiation Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	227.820, 39.500, 74.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.350 - 1.900
R-factor	0.2291
Rwork	0.228
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nqd
RMSD bond length	0.009
RMSD bond angle	1.347
Data reduction software	xia2 (3.4.2)
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.350	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	1.373
Rmeas	0.087	1.777
Rpim	0.051	1.112
Number of reflections	41762	2100
<I/σ(I)>	7.7	0.5
Completeness [%]	76.4	78.2
Redundancy	2.5	2.1
CC(1/2)	0.997	0.282

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	287.15	0.1M Sodium HEPES; MOPS (acid) 20% v/v Ethylene glycol; 10% w/v PEG 8000 0.03M Diethylene glycol; 0.03M Triethylene glycol; 0.03M Tetraethylene glycol; 0.03M Pentaethylene glycol 200uM DNA; 500uM AMPNPP; 2mM GTP; 2mM CoCl2; 140mM Monopotassium Glutamate