7P8Z

Short wilavidin biotin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.96546
Spacegroup name	P 21 21 2
Unit cell lengths	89.555, 67.384, 77.789
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.790 - 1.670
R-factor	0.1923
Rwork	0.189
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ouq
RMSD bond length	0.018
RMSD bond angle	2.169
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.790	1.695
High resolution limit [Å]	1.666	1.666
Rmerge	0.106
Number of reflections	55507	2756
<I/σ(I)>	10.1
Completeness [%]	99.6
Redundancy	6.5
CC(1/2)	0.998	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5-1.7 M ammonium sulfate 0.1 M HEPES at pH 7.5 2-3% PEG 400