7P8Y
Short wilavidin apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-02 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 89.675, 67.687, 77.512 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.710 - 2.180 |
| R-factor | 0.252 |
| Rwork | 0.249 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ouq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.866 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.710 | 2.266 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.129 | |
| Number of reflections | 25250 | 2805 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.6 | 4.6 |
| CC(1/2) | 0.993 | 0.984 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5-1.7 M ammonium sulfate 0.1 M HEPES at pH 7.5 2-3% PEG 400 |
