7P8L

Crystal structure of Pyrococcus abyssi 8-oxoguanine DNA glycosylase (PabAGOG) in complex with dsDNA containing cytosine opposite to 8-oxoG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	C 2 2 21
Unit cell lengths	62.256, 68.811, 140.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.170 - 1.250
R-factor	0.1364
Rwork	0.135
R-free	0.16100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7oy7
RMSD bond length	0.006
RMSD bond angle	0.904
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.170	1.270
High resolution limit [Å]	1.250	1.250
Rmerge	0.046	0.354
Number of reflections	83399	4101
<I/σ(I)>	28.5	5.3
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.999	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	Na acetate pH 4.5, KCl, MPD